The western blot (or protein immunoblot) is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein.
Save antibody by checking your blots before probing with our protein stains for membranes!
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| SpectraQuant™-HRP CL Spray chemiluminescent detection reagent serves as a highly sensitive substrate for detection of HRP enzyme conjugates in western blots | Western Block is used for blocking excess binding sites in ELISA, Western blotting, immunohistochemistry and other immunochemical applications. | SpectraQuant™-HRP CL chemiluminescent detection reagent serves as a highly sensitive substrate for detection of HRP enzyme conjugates in western blots |
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