The western blot (or protein immunoblot) is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein.
|SpectraQuant™-HRP CL Spray chemiluminescent detection reagent serves as a highly sensitive substrate for detection of HRP enzyme conjugates in western blots
|Western Block is used for blocking excess binding sites in ELISA, Western blotting, immunohistochemistry and other immunochemical applications.
|SpectraQuant™-HRP CL chemiluminescent detection reagent serves as a highly sensitive substrate for detection of HRP enzyme conjugates in western blots
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